ABSTRACT
Conjoined twins are very rare and the mortality rate of the fetus is extremely high. Early prenatal diagnosis is crucial, as it provides the opportunity for the mother and father to help in recognizing the conjunction of the twins and to help medical team in defining the prognosis of conjoined twins. We present a case of thoraco-omphalopagus conjoined twins diagnosed by two-dimensional and three-dimensional transabdominal sonography at 14(+2) weeks of gestation.
Subject(s)
Humans , Pregnancy , Fathers , Fetus , Mothers , Prenatal Diagnosis , Prognosis , Twins, ConjoinedABSTRACT
Lipomatous variant of angiomyofibroblastoma is a rare tumor of the vulva. Most of cases are usually premenopausal and present with a slowly growing mass without pain. A 46-year-old woman presented with a swelling of the left labium major. The lesion was thought to be a Batholin's gland cyst or a lipoma preoperatively and a local excision was performed. The tumor was well demarcated and rubbery. On microscopic examination, the alternating hypercellular and edematous hypocellular component with abundant vessels was noted. A large amount of adipose tissue was scattered in the lesion. Stromal cells were positive for vimentin, estrogen receptor, and progesterone receptor and negative for desmin antibody immunostaining. The local excision was enough for complete treatment. No recurrence was noted for 7 months after surgery.
Subject(s)
Female , Humans , Middle Aged , Adipose Tissue , Desmin , Estrogens , Lipoma , Receptors, Progesterone , Recurrence , Stromal Cells , Vimentin , VulvaABSTRACT
Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on 100microliter/ml laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.
Subject(s)
Bone Remodeling , Cell Movement , Collagen , Collagen Type I , Extracellular Matrix , Fibronectins , Fracture Healing , Glass , Hand , Integrins , Laminin , Ligands , Osteoblasts , Osteogenesis , Tissue EngineeringABSTRACT
To present a case of Percutaneous Endoscopic Jejunostomy in traumatic brain injured patient with subtotal gastrectomy. A 54-year-old man who injured a left subdural and intraventricular hemorrage by a falling. The patient had a history of the subtotal gastrectomy and suffered significant gastroesophageal reflux and recurrent aspiration pneumonia during nasogastric tubal feeding. The traditional Percutaneous Endoscopic Gastrostomy was inappropriate procedure for him because of significant alteration of gastric anatomy, insufficient volume of remnant stomach, and continuous gastroesophageal reflux. Direct percutaneous endoscopic placement of a jejunostomy tube was performed and we provide adequate enteral nutritional support in a traumatic brain injured patient with subtotal gastrectomy and gastroesophageal reflux.